Fig 1: A. Western blot analysis of AKT1, PDK2, FOXO1, FOXO3, PIK3R1 and, GAPDH for the untreated, GFP overexpressed, miR-96-5p overexpressed, miR-96-5p/antagomiR-96 overexpressed and, miR-96-5p/scrambled overexpressed mRPE cultures' whole-cell lysates. The quantity of proteins for FOXO1(D) and FOXO3(E) in untreated, GFP overexpressed and, miR-96-5p/antagomiR-96 overexpressed were reduced respected to the miR-96-5p overexpressed and miR-96-5p/scrambled overexpressed groups, while this was completely reversed for AKT1(B), PDK2(C) and, PIK3R1(F) proteins (full and non-adjusted images are provided in Supplementary Fig. S 8). The protein bands were quantified using Fiji software. The plot was drawn using GraphPad Prism 8. One-way ANOVA test was performed for data analysis. The test was performed in two independent experiments and the calculations were based on three independent determinations. Error bars represent means ± SD (*P < 0.05).